Repeat-associated centromere organization in <i>C</i>. <i>tropicalis</i>.

<p>(A) ChIP-seq analysis revealed that CENP-A and CENP-C bind to the <i>mid</i> core region in <i>C</i>. <i>tropicalis</i>. Here, the <i>x</i>-axis represents the structural components of a centromere in <i>C</i>. <i>tropicalis</i> and the <i>y</i>-axis represents the distribution of sequence reads of CENP-A (red) or CENP-C (green) ChIP DNA of the respective supercontig. Schematic representations of structural components of a centromere in <i>C</i>. <i>tropicalis</i> are shown below each ChIP-seq reads. Black boxes represent the <i>mid</i> regions, blue arrows indicate inverted repeats, the left repeat (<i>LR</i>) and the right repeat (<i>RR</i>). Scale bar, 2 kb. (B) ChIP-qPCR assays confirm the binding of kinetochore proteins across the centromere in Scnt 8. The <i>x</i>-axis represents coordinates on the supercontigs and the <i>y</i>-axis denotes the qPCR value as a percentage of the total chromatin input with standard error mean (SEM). Scale bar, 2 kb (C) Sequence conservation between the <i>mid</i> core regions (<i>mid</i>s) and inverted repeats (<i>IR</i>s) from different centromeres in <i>C</i>. <i>tropicalis</i>. Homology is calculated as the percentage of aligned nucleotides in a pair-wise alignment, measured from the shorter sequence. On the other hand, identity is the percentage of aligned and conserved nucleotides in the pair-wise alignment, again measured from the shorter sequence. Averages are calculated from all pair-wise alignments, weighted by length. <i>IR</i>* and <i>IR</i># denote average value with respect to either others or self respectively.