Overexpression of K_v3.3 did not have a clear effect on hemin-induced erythroid differentiation.

<p>mRNA (A) and protein (B) expression levels of K_v3.3 were increased in the K562 overexpressed cell line compared to the normal K562 cell line. The relative mRNA expression of the K_v3.3 was normalized to the GAPDH gene and expressed as a fold change relative to the control group. The relative protein expressions of the Kv3.3 were expressed as a fold change relative to the control. (C) The degree of hemin-induced erythroid differentiation was measured using benzidine staining in K_v3.3-overexpressed K562 cells or normal K562 cells. There was no significant difference between the two, however. (D) Hemoglobin quantification demonstrated that overexpressed K_v3.3 did not have any effect on hemin-induced K562 erythroid differentiation.