Cmr1 does not regulate histone occupancy or cotranscriptional histone modifications.

<p>WT and <i>cmr1</i>Δ strains were treated with SM to induce <i>ARG1</i> transcription and occupancies of histone H3 and histone modifications were determined by ChIP. A) H3 ChIP occupancies at <i>ARG1</i> and <i>HIS4</i> in SM-induced WT and <i>cmr1</i>Δ cells, and in the non-induced WT cells are shown. B) Occupancies of histone H3 modifications (acetylation, Ac; methylation, me; 2, di; 3, tri) normalized to H3 occupancy at <i>ARG1</i> in SM-induced WT and <i>cmr1</i>Δ strains are shown. The levels of these modifications for an un-induced WT strain are also shown. C) Trimethylated (me3) H3K4 and K3K36 occupancies normalized to H3 levels at <i>HIS4</i> ORFs in the WT and <i>cmr1</i>Δ cells. D) Whole cell extracts were prepared from WT and <i>cmr1</i>Δ cells and H3 acetylation levels were detected by western blot. H3 and Gcd6 (a translational factor) were used as loading controls.