Comparison between HTRF and ELISA insulin detection assays.

<p>Supernatants collected from glucose-stimulated INS-1E cells (20 mM glucose, 90 min, 37°C) were measured concurrently with HTRF or ELISA insulin assays. The respective HTRF and ELISA assay-derived insulin concentration values were plotted. A linear regression curve of the data showed close correlation of the insulin values from the two methods (slope = 1.15 ± 0.16, R² = 0.84). Results are represented as mean insulin concentrations ± SEM performed in triplicate in 3 independent experiments.