Establishment of solitary liver-metastasis model.
- Shuya Yano (157763)
- Kiyoto Takehara (2157013)
- Shinji Miwa (292708)
- Hiroyuki Kishimoto (200624)
- Yukihiko Hiroshima (568068)
- Takashi Murakami (138867)
- Yasuo Urata (157765)
- Shunsuke Kagawa (157766)
- Michael Bouvet (483662)
- Toshiyoshi Fujiwara (157767)
- Robert M. Hoffman (302618)
Publication date
February 2016
DOIAbstract
<p><b>A.</b> Scheme for establishing solitary live metastasis. <b>B.</b> HCT-116L3-RFP cells (2×10<sup>5</sup>), in Matrigel (BD), were inoculated in the subserosa space of the liver of nude mice (5 weeks old). Panels 1, 2. Open abdominal wall. Panel 3. Exteriorizing the liver. Panel 4. Injection of HCT-116L3-RFP cells in the subserosa of the liver. Panel 5. RFP-fluorescence image of injected HCT-116L3-RFP cells in the subserosa of the liver. Panel 6. Merged image of injected HCT-116L3-RFP cells in the subserosa of the liver. <b>C.</b> Time-course imaging of liver metastasis growth. Upper panels; low magnification images. Lower panels; high magnification images. <b>D.</b> Experimental flowchart with endpoints.</p
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