SftA is not required for rescue of spore formation by <i>spoIIIEΔγ</i> intragenic suppressor mutations.

<p>Strains were induced to sporulate for 24–36 h in DSM medium, unsporulated cells were eliminated by heat-kill, and the number of spores was measured by plating for cfu. Sporulation levels are similar for <i>sftA+</i> and Δ<i>sftA</i> strains bearing <i>spoIIIEΔγ</i> mutant alleles: P260L (BOSE2286; BOSE2492), E312A (BOSE2121; BOSE2486), Y316D (BOSE2284; BOSE2490), P319S (BOSE2321; BOSE2496), A343V (BOSE2288; BOSE2494), E347G (BOSE2323; BOSE2498), P492Q (BOSE2120; BOSE2935), and T617A (BOSE2123; BOSE2488). Sporulation efficiencies for strains harboring full-length (“f.l.”) <i>spoIIIE</i> (bKM776) and <i>spoIIIEΔγ</i> (BOSE2042) are plotted for comparison. The average of at least 3 replicates is plotted. Error bars indicate one standard deviation in each direction.