Detection of <i>H</i>. <i>pylori SS1</i> in slides, by fluorescence <i>in situ</i> hybridization (FISH) using the Cy3_HP_LNA/2OMe_PS probe at different pH values, analyzed by epifluorescence microscopy.

<p>A to C—FISH protocol performed on smears of pure cultures of <i>H</i>. <i>pylori</i> SS1 incubated with 0.2 μM of probe. D to F—FISH protocol on smears of <i>H</i>. <i>pylori</i> SS1 cultures without probe used as negative control. A and D—Experiments performed at pH 2. B and E—Experiments performed at pH 4. C and F—Experiments performed at pH 7. All images were taken at equal exposure times. Scale bar: 10 μm.