MOESM4 of Chicken embryonic stem cells and primordial germ cells display different heterochromatic histone marks than their mammalian counterparts

Additional file 4. Nuclear distribution of histone post-translational modifications of active chromatin in chicken ESCs, RA-differentiated ESCs, BCs, PGCs and CEFs. (A) Immunodetection of H3K4me3 (a–e) and DNA counterstaining with TO-PRO-3 (f–j) in nuclei of ESCs, RA-differentiated ESCs, BCs, PGCs and CEFs. Overlay of H3K4me3 (green) and TO-PRO-3 (magenta) is shown below (k–o). (B) Immunodetection of H3K9ac (a–e) and DNA counterstaining with TO-PRO-3 (f–j) in nuclei of ESCs, RA-differentiated ESCs, BCs, PGCs and CEFs. Overlay of H3K9ac (green) and TO-PRO-3 (magenta) is shown below (k–o). Single confocal sections of representative nuclei are shown. Scale bar 5 μm