Quantitative analysis of hepatocyte functions.

<p>(A) Albumin secretion and (B) urea synthesis of cultured hepatocytes on day 9 were measured using ELISA and colorimetric assay, respectively. “Hepatocyte co-culture” indicates NIH-3T3 J2 co-cultured samples, “(+) F-CM”: hepatocyte monoculture supplemented with F-CM (55 mg/ml); “(+) concentrated media”: hepatocyte monoculture supplemented with concentrated media (unconditioned). Results were analyzed using one-way ANOVA and Tukey’s <i>post hoc</i> test. Bars: ± 1 SD, <i>n</i> = 9. Bars labeled with different letters (a,b,c and d) are significantly different (Tukey’s test, P<0.01)