Microglia after CLP demonstrate distinct transcriptional differences, despite absence of morphological signs of activation.

<p>There is no difference in intensity of Iba1 immunoreactivity in somatosensory cortex among unoperated (A), sham operated (B) and post-CLP mice (C) 14 days after operation (E). There is also no difference in Iba1 immunoreactivity of microglia in regions of somatosensory cortex with evidence of vasculopathy (IgG deposition, D) compared to regions without IgG immunoreactivity (C, F). Despite the absence of activated amoeboid morphology, microglia in 14 day post-CLP mice demonstrate a distinct transcriptional profile compared to both sham operated and unoperated controls (G).