The effect of dcEF on the migration of BC and LC cells.

<p><b>(A)</b> Composite trajectories of BC and LC cells migrating in the absence and in the presence of dcEFs are shown as circular diagrams. In each diagram, the initial point of each trajectory was placed at the center of the circle. The x-axis corresponds to the direction of the electric field. The cathode was always placed at the right side of the diagram. Each trajectory was constructed from 120 (BC) or 48 (LC) successive positions of cell centroids recorded at 15-sec (BC) or 150-sec (LC) time intervals, immediately after the exposure of cells to dcEF. The movement of BC and LC was recorded for 30 minutes and 150 minutes, respectively. (<b>B</b>) Diagrams presenting the values of directional cosines γ for BC and LC cells depending on applied field strength; mean ± SEM; p<0.05 vs 0 V/cm (<b>C</b>) The dynamics of reversibility of the direction of cell movement. The directionality of cell movement was completely reversible upon reversing the field polarity (3V/cm). For both cell sublines the average directional cosines γ were analyzed every 5 minutes. Perpendicular lines indicate the time when the electric field polarity was reversed (n = 50). *Statistically significant vs. 0 V/cm (p<0.05).