<p>Live-cell imaging of PST02549-GFP and TaEDC4-mCherry in <i>N</i>. <i>benthamiana</i> leaf cells. Images show a single optical section of 0.8 μm. The white asterisk indicates a pavement cell expressing only the TaEDC4-mCherry fusion, in which no large P-body was detected. Proteins were transiently expressed in <i>N</i>. <i>benthamiana</i> leaf cells by agroinfiltration. Live-cell imaging was performed with a laser-scanning confocal microscope with a sequential scanning mode two days after infiltration. GFP and the chlorophyll were excited at 488 nm; the mCherry was excited at 561 nm. GFP (green), mCherry (red), and chlorophyll (blue) fluorescence were collected at 505–525 nm, 580–620 nm and 680–700 nm, respectively. Black arrowheads indic...
<p>(<b>A</b>) Expression of SBT3.3-mCherry (50 hpi) results in a uniform extracellular fluorescence....
<p>Constructs expressing full-length SnRK1, ADK, DCL4, or DRB4 proteins fused to the N- or C-termina...
<p>(<b>A</b>) Observations of GFP fluorescence in various cells and tissues of the stable transgenic...
<p>(A) Schematic representation of the protein primary structure of AtEDC4, NbEDC4, and TaEDC4. Numb...
<p>(A) Categorical scatterplots showing the diameter of P-bodies labelled by PST02549-GFP and/or TaE...
<p>(A) Schematic representation of the protein primary structure of PST15391 and PST18447. Yellow: p...
<p>(a, c-h) Fluorescent proteins observed in leaves from plants resulting from crosses between <i>pS...
<p>Visualization of soluble GFP or PP2-A1 tagged with GFP or CFP in leaves from <i>pSUC2</i>:<i>PP2-...
<p>A) The fluorescence observation of control GFP protein in Arabidopsis protoplasts. Bar = 10 µm. ...
(A) Full length WT-RTP sequence and the RTP sequence from mutant Mut-Δ5670–5685 (RTP-Δ5AA) were fuse...
<p>(A) Subcellular localization of NtRPT6-GFP and XopJ-mCherry fusions in <i>N. benthamiana</i> leav...
(A) Cellular localization of GFP fluorescence in mature N. benthamiana leaves agroinfiltrated with a...
<p>Fluorescent proteins observed in leaves from plants carrying GFP expressed in different subcellul...
<p>Fluorescent proteins observed in leaves from plants carrying GFP expressed in different subcellul...
Confocal microscopy of lower epidermal cells of GFP-TUA6 stable transgenic N. benthamiana leaves. Le...
<p>(<b>A</b>) Expression of SBT3.3-mCherry (50 hpi) results in a uniform extracellular fluorescence....
<p>Constructs expressing full-length SnRK1, ADK, DCL4, or DRB4 proteins fused to the N- or C-termina...
<p>(<b>A</b>) Observations of GFP fluorescence in various cells and tissues of the stable transgenic...
<p>(A) Schematic representation of the protein primary structure of AtEDC4, NbEDC4, and TaEDC4. Numb...
<p>(A) Categorical scatterplots showing the diameter of P-bodies labelled by PST02549-GFP and/or TaE...
<p>(A) Schematic representation of the protein primary structure of PST15391 and PST18447. Yellow: p...
<p>(a, c-h) Fluorescent proteins observed in leaves from plants resulting from crosses between <i>pS...
<p>Visualization of soluble GFP or PP2-A1 tagged with GFP or CFP in leaves from <i>pSUC2</i>:<i>PP2-...
<p>A) The fluorescence observation of control GFP protein in Arabidopsis protoplasts. Bar = 10 µm. ...
(A) Full length WT-RTP sequence and the RTP sequence from mutant Mut-Δ5670–5685 (RTP-Δ5AA) were fuse...
<p>(A) Subcellular localization of NtRPT6-GFP and XopJ-mCherry fusions in <i>N. benthamiana</i> leav...
(A) Cellular localization of GFP fluorescence in mature N. benthamiana leaves agroinfiltrated with a...
<p>Fluorescent proteins observed in leaves from plants carrying GFP expressed in different subcellul...
<p>Fluorescent proteins observed in leaves from plants carrying GFP expressed in different subcellul...
Confocal microscopy of lower epidermal cells of GFP-TUA6 stable transgenic N. benthamiana leaves. Le...
<p>(<b>A</b>) Expression of SBT3.3-mCherry (50 hpi) results in a uniform extracellular fluorescence....
<p>Constructs expressing full-length SnRK1, ADK, DCL4, or DRB4 proteins fused to the N- or C-termina...
<p>(<b>A</b>) Observations of GFP fluorescence in various cells and tissues of the stable transgenic...