Thrombin generation curves and parameters in zebrafish, effect of temperature and comparison with humans.

<p>Thrombin generation was measured in zebrafish at 28°C and 37°C and compared to thrombin generation in humans at 37°C with low (no tissue factor (TF) added) or high (100 pM) TF. <b>(A)</b> Thrombin generation curves are shown (n = 8 or 9 for fish, n = 3 for humans). <b>(B)</b> Mean thrombin generation parameters and their CV are calculated. Statistical differences as compared to the zebrafish group measured at 37°C are indicated (*p<0.05, **p<0.01, ***p<0.0001, 1-way ANOVA). <b>(C-D)</b> Thrombin generation was measured in 6 independent experiments, in a total of 34 zebrafish. <b>(C)</b> Thrombin generation curves are shown. Each color represents an independent experiment. <b>(D)</b> Mean thrombin generation parameters and their CV are calculated, as well as the CV between the means of all 6 experiments (interexp. CV).