<i>ubr2Δ</i> and <i>dsn1-S240D/S250D</i> phospho-mimic mutants do not function epistatically.

<p>Quantitation of Mtw1-YFP levels at kinetochore foci in wild type (black), <i>psh1Δ</i> (blue), <i>ubr2Δ</i> (green) and <i>psh1Δ ubr2Δ</i> (red): Fluorescence intensity levels are normalised relative to wild-type mean intensity. Top panels and bottom panels display the distribution of intensities and the mean intensity ± standard deviation, respectively. (A) Strains contain wild type <i>DSN1</i>: <i>PSH1 UBR2</i> 1.00±0.14, <i>psh1Δ UBR2</i> 1.04±0.18, <i>PSH1 ubr2Δ</i> 1.07±0.24, <i>psh1Δ ubr2Δ</i> 1.24±0.2***. (B) Strains contain the <i>dsn1-S240D/S250D</i> allele: <i>PSH1 UBR2</i> 1.00±0.15, <i>psh1Δ UBR2</i> 1.08±0.16, <i>PSH1 ubr2Δ</i> 1.19±0.24, <i>psh1Δ ubr2Δ</i> 1.48±0.27***. ***<i>p</i>-value <0.0001 is a <i>t</i> test, comparing the levels of Mtw1-YFP in <i>PSH1 UBR2</i> cells with <i>psh1Δ ubr2Δ</i> double mutant cells (both for wild-type <i>DSN1</i> and for the <i>dsn1-S240D/S250D</i> strain). For <i>psh1Δ ubr2Δ</i> cells the <i>dsn1-S240D/S250D</i> mutant had higher levels of Mtw1-YFP than the DSN1 (1.24±0.2 <i>vs</i>. 1.48±0.27 <i>p</i>-value <0.0001).