Representative photomicrographs of liver sections stained with picrosirius red.

<p>Five animals per group and three H&E sections per animal were analysed. (A) Negative control group livers had weak staining limited to centrilobular veins (left panel) and the portal tract (right panel). (B) Liver sections from the alcohol-only group exhibited marked portal fibrosis (left panel) and staining between the hepatic cords (right panel). (C) The alcohol-CARV 5 mg/kg group livers had a weak fibrotic response limited to centrilobular veins (left panel) and portal tract (right panel). (D) Morphometric quantification of Sirius red stained areas demonstrated an attenuation of the fibrotic process in the alcohol-CARV 5 mg/kg group compared to the alcohol-only group. (E) The area fraction of total fibrosis, including fibrosis in the portal tract area, in rats with alcohol-induced liver injury in relation to the Ishak score. The alcohol-CARV 5 mg/kg group livers had an attenuation of the fibrotic process. PT, portal tract; CV, central vein; asterisk, hepatic cords. Magnification 400×, scale bar = 50 μm. (**<i>p</i> < 0.01, *<i>p</i> < 0.05, Kruskal-Wallis test followed by Dunn’s test).