Defective heterochromatin in <i>dhp1</i> mutants is marked by reduced H3K9 methylation at reporter genes.

<p>(A) Ten-fold serial dilution on media containing 20 μg/ml TBZ demonstrates chromosome segregation defects in <i>dhp1-1</i> mutants. (B-C) Chromatin immunoprecipitation (ChIP) performed with an anti-H3K9me₂ antibody shows reduced H3K9me₂ enrichment in <i>dhp1-1</i> at the reporter genes located at pericentromeric region (<i>otr</i>, B) and <i>mat2</i> (C). (D-E) The same reduction was not observed at the centromeric (D) and mating type locus (E) repeat regions by H3K9me2 ChIP. Relative enrichment (normalized to input) gives the approximate ratio between H3K9me₂ levels on the indicated heterochromatic regions versus the euchromatic control <i>leu1</i>^<i>+</i>. * <i>p</i> ≤ 0.05 as determined by Student’s <i>t</i> test comparing the indicated sample values with wt values.