Time course of differentiation into osteoblastic lineage.

<p>(A) Schematic representation of the conditions. hFOB cells were expanded and maintained at 34°C in basal media (BM), thereafter designated as control cells. Cells were shifted to 39°C in BM or in Osteogenic Induction Media (OIM). Samples were collected at times indicated by the arrows. (B) Alizarin Red S staining of extracellular matrix deposition in control cells (left) and cells grown in OIM (right) for 8 days. Scale bars indicate 10 μm at 20X magnification. (C) Expression of OB related genes by qPCR in cells exposed to BM (blue) or OIM (red) at 39°C on days 0, 3, 5 and 8. The panels correspond to Collagen 1A1 (COL1A1), Alkaline Phosphatase Liver/Bone/Kidney (ALP), and Runt-related transcription factor 2 (RUNX2). All values were normalized to control cells and SD calculated based on triplicate samples. One of more than 3 independent experiments is shown here.