Additional file 6: Figure S5. of Development of 5‘ LTR DNA methylation of latent HIV-1 provirus in cell line models and in long-term-infected individuals

5’ LTR CpG methylation of the HIV-1 provirus in the latent reservoir of infected patients who were treated for up to 3 years. (A) 5’ LTR methylation profiles of HIV-1-infected individuals, nos. 1 to 15, are presented. Individual no. 3 was analyzed after 20 and 30 months of the therapy. The methylation levels are presented as a mean percentage of methylated CpGs (mCpGs) in HIV-1 promoters. An analysis of promoter molecules is shown as a linear array of open circles representing non-methylated CpG residues and closed circles representing methylated CpG residues. Each line represents one sequenced molecule of the 5’ LTR. The rectangle schematically represents the 5’ LTR region U3 with a distribution of individually analyzed CpG dinucleotides and transcription factor binding sites. (B) Levels of HIV-1 5’ LTR methylation in patients who were treated for up to 3 years. Each diamond represents one patient, numbers of patients are indicated corresponding to Additional file 5: Table S1. The time (months) spent undergoing therapy is depicted on the x-axis whereas the percentage of methylated CpGs in the HIV-1 5’ LTR in the latent reservoir of infected patients is depicted on the y-axis. The methylation levels are presented as a mean percentage of methylated CpGs in HIV-1 promoters. (TIF 539 kb