Additional file 4: Figure S4. of Development of 5‘ LTR DNA methylation of latent HIV-1 provirus in cell line models and in long-term-infected individuals

mRNA expression of histone deacetylases in the 2D12 cell line. HDAC1, HDAC2, HDAC3, and HDAC8 relative mRNA levels in the non-stimulated 2D12 cells and 2D12 cells stimulated for 24 h with TNF-α and PMA were determined by qRT-PCR. mRNA expression was normalized to the expression of RNA polymerase II, polypeptide A (POLR2A) housekeeping gene. Data are presented as a mean ± SD of triplicates. The p values were calculated by the non-paired Student’s t test. Significance was assigned as follows: ** for p values <0.01, *** for p values