MCDD-driven NAFLD alters hepatic levels of IL-17A and IL-17F production.

<p>8 week old male C57BL/6 mice (<i>n</i> = 4/condition) were placed on MCDD or chow diet for 4 weeks. (<b>A</b>) IL-17A and IL-17F production was determined in cells following the gating on: CD45⁺, TCRβ⁺, NK1.1^-, CD4⁺ or CD8⁺. (<b>B</b>) Percent of hepatic immune cells (CD45⁺) producing IL-17A. (<b>C</b>) Percent of hepatic immune cells (CD45⁺) producing IL-17F. (<b>D</b>) Percent of hepatic TCRβ⁺ cells (CD4⁺ or CD8⁺ T cells) producing IL-17A. (<b>E</b>) Percent of hepatic TCRβ⁺ cells (CD4⁺ or CD8⁺ T cells) producing IL-17F. (<b>F</b>) Mean fluorescent intensity of IL-17A in TCRβ⁺ cells (CD4⁺ or CD8⁺ T cells). (<b>G</b>) Mean fluorescent intensity of IL-17F in TCRβ⁺ cells (CD4⁺ or CD8⁺ T cells). (<b>H</b>) Percent of hepatic immune cells (CD45⁺) expressing GR-1 and CD11b. Data represent means + SE; a representative of two separate experiments. Student <i>t</i> test **<i>P</i> < 0.01.