Effects of SMO inhibition on Tat-induced gene expression in SH-SY5Y cells.

<p>Cells grown on 35-mm-diameter tissue culture dishes were overnight pretreated with CHL (10 nM) or medium alone before the addition of Tat (200 ng/ml) for 4 h. After incubation at 37°C, the cells were homogenized and total RNA has been purified to assess mRNA levels of NQO1, CAT, SOD1, SOD2, HO-1 genes by RT-qPCR. Data are calculated relative to GAPDH and are expressed as the mean fold change compared with control. Each value represents the mean ± SEM of three independent experiments. One-way ANOVA, followed by Bonferroni's test, was used to determine significant differences. NQO1: * p≤0.01 vs CTRL, ^ p≤0.01 vs Tat; CAT: * p≤0.01 vs CTRL, ^ p≤0.01 vs Tat; SOD1: * p≤0.01 vs CTRL, ^ p≤0.01 vs Tat; SOD2: * p≤0.01 vs CTRL, ^ p≤0.01 vs Tat; HO-1: * p≤0.01 vs CTRL, ^ p≤0.01 vs Tat.