Tumorigenic assay of rhabdosphere cells. A) FACS analysis of CD133 positive cells in adherent and in rhabdosphere cells (SC-m SPH ) before subcoutaneously injection (pre-injection); B) histogram showing the tumor development and volumes of xenografts from adherent (Ad) or rhabdosphere cells (SC-m SPH ), (n = 6, *p > 0,05). (TIF 341 kb
Figure S4. siRNA-mediated knockdown of HDAC3 in eRMS and aRMS. Analysis of MHC expression in RD, Rh3...
Figure S5. Representative immunohistochemistry for CD68 of mouse eRMS tissue and primary cells. Necr...
Figure S1. Expression of CSC-associated genes up-regulated in AB12 and RN5 cells after cisplatin tre...
CD133 positive population in primary (first generation) and secondary (second generation) rhabdosphe...
Histogram of rhabdospheres formation dependent on number of cells. RD seeded at 500 or 1,000 cells/w...
Results of FACS analysis of the levels of CD90, CD117 and CD105 positive populations, which remain u...
Radiation (RT) or/and 2 μM U0126 were provided after RD rhabdophere spheres formation. Analysis of c...
Persistent phospho-ERK1/2 (ERK1/2-P) down regulation by U0126. Western blot analysis of protein lysa...
The identification of signaling pathways that affect the cancer stem-like phenotype may provide insi...
Figure S1. Phosphohistone H3 (pHH3) expression and rhabdomyoblast count in VCR and ENT + VCR-treated...
Figure S3 (A) RPPA-TCGA elaboration of E-Cadherin and Fibronectin expressions. Data were obtained fr...
Figure S2. GFP-positive cancer cells in ID8 and ID8-KRAS cells in vivo. Mice were i.p. injected with...
Figure S3. CRISPR/Cas9 mediated HDACs inhibition and evaluation of tumor cell growth inhibition in e...
Figure S1. KRAS activation by evaluating the downstream protein of RAS cascade. ID8-GFP and ID8-KRAS...
Table S1. KEGG pathway analysis for genes upregulated in ID8-KRAS-3D cells compared to ID8-3D cells....
Figure S4. siRNA-mediated knockdown of HDAC3 in eRMS and aRMS. Analysis of MHC expression in RD, Rh3...
Figure S5. Representative immunohistochemistry for CD68 of mouse eRMS tissue and primary cells. Necr...
Figure S1. Expression of CSC-associated genes up-regulated in AB12 and RN5 cells after cisplatin tre...
CD133 positive population in primary (first generation) and secondary (second generation) rhabdosphe...
Histogram of rhabdospheres formation dependent on number of cells. RD seeded at 500 or 1,000 cells/w...
Results of FACS analysis of the levels of CD90, CD117 and CD105 positive populations, which remain u...
Radiation (RT) or/and 2 μM U0126 were provided after RD rhabdophere spheres formation. Analysis of c...
Persistent phospho-ERK1/2 (ERK1/2-P) down regulation by U0126. Western blot analysis of protein lysa...
The identification of signaling pathways that affect the cancer stem-like phenotype may provide insi...
Figure S1. Phosphohistone H3 (pHH3) expression and rhabdomyoblast count in VCR and ENT + VCR-treated...
Figure S3 (A) RPPA-TCGA elaboration of E-Cadherin and Fibronectin expressions. Data were obtained fr...
Figure S2. GFP-positive cancer cells in ID8 and ID8-KRAS cells in vivo. Mice were i.p. injected with...
Figure S3. CRISPR/Cas9 mediated HDACs inhibition and evaluation of tumor cell growth inhibition in e...
Figure S1. KRAS activation by evaluating the downstream protein of RAS cascade. ID8-GFP and ID8-KRAS...
Table S1. KEGG pathway analysis for genes upregulated in ID8-KRAS-3D cells compared to ID8-3D cells....
Figure S4. siRNA-mediated knockdown of HDAC3 in eRMS and aRMS. Analysis of MHC expression in RD, Rh3...
Figure S5. Representative immunohistochemistry for CD68 of mouse eRMS tissue and primary cells. Necr...
Figure S1. Expression of CSC-associated genes up-regulated in AB12 and RN5 cells after cisplatin tre...