Daphmacromines A–J, Alkaloids from <i>Daphniphyllum macropodum</i>

Ten new yuzurine-type <i>Daphniphyllum</i> alkaloids, daphmacromines A–J (<b>1</b>–<b>10</b>), along with seven known alkaloids were isolated from the leaves and stems of <i>Daphniphyllum macropodum</i>. Their structures were elucidated by extensive spectroscopic techniques, including 2D NMR spectroscopy and mass spectrometry, and the structure of <b>1</b> was confirmed by single-crystal X-ray diffraction. The pesticidal and cytotoxic activities of the isolated alkaloids were evaluated in vitro against brine shrimp (<i>Artemia salina</i>) and five human cancer cell lines (HL-60, SMMC-7721, A-549, MCF-7, and SW480), respectively. This study also suggested structural revisions of oxodaphnigracine, oxodaphnigraciline, and epioxodaphnigraciline