Example datasets of confocal smFRET experiments in Photon-HDF5 format

<p>This fileset contains freely-diffusing single-molecule FRET datasets on the Photon-HDF5 file format (http://photon-hdf5.org/). These files are also used as demo datasets for the FRETBursts burst analysis software (http://tritemio.github.io/FRETBursts).</p> <p>These datasets come from smFRET experiments on a us-ALEX, ns-ALEX and 8-spot smFRET setups.</p> <p> </p> <p><strong>File format</strong></p> <p>The file format is Photon-HDF5, an open binary format for single-molecule data: </p> <p> http://photon-hdf5.org/</p> <p> </p> <p><strong>Dataset description</strong></p> <p>Currently, two datasets are published: the first is a single-spot usALEX dataset and the second one is an 8-spot smFRET with single laser excitation.</p> <p><strong>usALEX: '0023uLRpitc_NTP_20dT_0.5GndCl.hdf5'</strong></p> <p>This dataset is a usALEX measurement of 20dt ssDNA oligos doubly labeled with Cy3B and Atto647N. The excitation laser frequencies are 523nm and 635nm. The objective lens is a 1.2NA Olympus water immersion lens. The 2 detectors are both Perkin Elmer (now Excelitas) single-pixel SPADs.</p> <p><strong>8-spot smFRET: '12d_New_30p_320mW_steer_3.hdf5'</strong></p> <p>This dataset is a smFRET measurement performed on a 8-spot smFRET system employing a single laser excitation (8ps pulsed, 532nm).</p> <p>The setup is an 8 excitation spots smFRET setup employing 2 8-pixel SPAD arrays fabricated in custom technology by the SPADLab group at Politecnico di Milano (Italy). The 8 excitation spots are generated by an LCOS-SLM and focused in the sample with an water immersion NA 1.2 Olympus lens.</p> <p>The sample is 40 base-pairs dsDNA oligo doubly labeled with ATTO550 and ATTO647N. The base-pair separation between the dyes is 12 base-pairs.