Label-Free Quantitative LC–MS Proteomics of Alzheimer’s Disease and Normally Aged Human Brains
- Victor P. Andreev (402750)
- Vladislav A. Petyuk (218084)
- Heather M. Brewer (154798)
- Yuliya V. Karpievitch (259460)
- Fang Xie (103933)
- Jennifer Clarke (122874)
- David Camp (1669342)
- Richard D. Smith (39311)
- Andrew P. Lieberman (201159)
- Roger L. Albin (316830)
- Zafar Nawaz (96166)
- Jimmy El Hokayem (2078962)
- Amanda J. Myers (2078959)
DOIAbstract
Quantitative proteomics analysis of cortical samples of 10 Alzheimer’s disease (AD) brains versus 10 normally aged brains was performed by following the accurate mass and time tag (AMT) approach with the high resolution LTQ Orbitrap mass spectrometer. More than 1400 proteins were identified and quantitated. A conservative approach of selecting only the consensus results of four normalization methods was suggested and used. A total of 197 proteins were shown to be significantly differentially abundant (<i>p</i>-values <0.05, corrected for multiplicity of testing) in AD versus control brain samples. Thirty-seven of these proteins were reported as differentially abundant or modified in AD in previous proteomics and transcriptomics publications...
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