The 1,3-dipolar cycloaddition of azides with ring-strained alkynes is one of the few bioorthogonal reactions suitable for specific biomolecule labeling in complex biological systems. Nevertheless, azide-independent labeling of proteins by strained alkynes can occur to a varying extent, thereby limiting the sensitivity of assays based on strain-promoted azide–alkyne cycloaddition (SPAAC). In this study, a subset of three cyclooctynes, dibenzocyclooctyne (DIBO), azadibenzocyclooctyne (DIBAC), and bicyclo[6.1.0]nonyne (BCN), was used to evaluate the azide-independent labeling of proteins in vitro. For all three cyclooctynes, we show that thiol-yne addition with reduced peptidylcysteines is responsible for most of the azide-independent polypep...
The ability to study biomolecules within their naturally occurring context is often complicated by t...
Chemical transformations that join two molecular components together rapidly while remaining highly ...
This document is the unedited Author’s version of a Submitted Work that was subsequently accepted fo...
Contains fulltext : 94017.pdf (publisher's version ) (Closed access
An enrichment strategy was devised for azide derivatized macromolecules, based on strain-promoted al...
A nearly forgotten reaction discovered more than 60 years ago—the cycloaddition of a cyclic alkyne a...
The strain-promoted alkyne-azide cycloaddition (SPAAC) is the most commonly employed bioorthogonal r...
Little is known about the reactivity of strain-promoted alkyne–azide cycloaddition (SPAAC) reagents ...
Contains fulltext : 92236.pdf (publisher's version ) (Closed access)4 p
Protein sulfenic acids are formed by the reaction of biologically relevant reactive oxygen species w...
Strained alkynes, i.e., alkynes distorted from the ideal linear geometry, undergo cycloaddition reac...
\u3cp\u3eIntroduction: We report on our evaluation of the strain-promoted cyclooctyne-azide cycloadd...
The site specific attachment of the reactive TMTHSI-click handle to the N-terminus of peptides and p...
ABSTRACT: Protein sulfenic acids are formed by the reaction of biologically relevant reactive oxygen...
Bioorthogonal chemistry allows rapid and highly selective reactivity in biological environments. The...
The ability to study biomolecules within their naturally occurring context is often complicated by t...
Chemical transformations that join two molecular components together rapidly while remaining highly ...
This document is the unedited Author’s version of a Submitted Work that was subsequently accepted fo...
Contains fulltext : 94017.pdf (publisher's version ) (Closed access
An enrichment strategy was devised for azide derivatized macromolecules, based on strain-promoted al...
A nearly forgotten reaction discovered more than 60 years ago—the cycloaddition of a cyclic alkyne a...
The strain-promoted alkyne-azide cycloaddition (SPAAC) is the most commonly employed bioorthogonal r...
Little is known about the reactivity of strain-promoted alkyne–azide cycloaddition (SPAAC) reagents ...
Contains fulltext : 92236.pdf (publisher's version ) (Closed access)4 p
Protein sulfenic acids are formed by the reaction of biologically relevant reactive oxygen species w...
Strained alkynes, i.e., alkynes distorted from the ideal linear geometry, undergo cycloaddition reac...
\u3cp\u3eIntroduction: We report on our evaluation of the strain-promoted cyclooctyne-azide cycloadd...
The site specific attachment of the reactive TMTHSI-click handle to the N-terminus of peptides and p...
ABSTRACT: Protein sulfenic acids are formed by the reaction of biologically relevant reactive oxygen...
Bioorthogonal chemistry allows rapid and highly selective reactivity in biological environments. The...
The ability to study biomolecules within their naturally occurring context is often complicated by t...
Chemical transformations that join two molecular components together rapidly while remaining highly ...
This document is the unedited Author’s version of a Submitted Work that was subsequently accepted fo...