<p>Overexpression of a perturbation protein was used to test for degradation effects on Ntag-RFP. Overall degradation is reduced with the target/perturbation plasmid system as indicated by the control conditions (Δ<i>clpP</i> + pTrc versus WT + pTrc). ClpA overexpression did not change degradation detectably. Overexpression of each ClpP and ClpS enhanced the degradation rate. Single * designates p < 0.05 and triple (***) designates p < 0.001. Calculations of p-values are between Δ<i>clpP</i> + pTrc and the given condition. Values are mean ± s.e.m. (n = 4 to 6).</p
The major chaperones identified in Escherichia coli that assist in protein folding include trigger f...
The ClpS adaptor collaborates with the AAA+ ClpAP protease to recognize and degrade N-degron substra...
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2009.This electronic versi...
<p>(A) Schematic representation of the inducible degradation system used to inducibly deplete ClpP2 ...
<p>(A) Quantitative PCR to determine transcript levels of over-represented proteins upon depletion o...
Targeted protein degradation is crucial for the correct function and maintenance of a cell. In bacte...
Staphylococcus aureus is an important pathogen, causing a wide range of infections including sepsis,...
ClpP is a self-compartmentalizing protease with crucial roles in bacterial and mitochondrial protein...
<p>(A) Schematic presentation of the Clp1 fusion gene, which is expressed under the control of <i>GA...
<p>A. Alignment of FtsZ residues 349 through 358 from the linker domain with the C-terminal ClpX rec...
Conditional degradation of proteins by ATP-dependent proteases is an important regulatory principe....
This article contains supporting information online at www.pnas.org/cgi/content/full/ 0910484106/DC...
Intracellular proteolysis is an essential regulatory process that affects cellular physiology. Since...
<p>ClpX and ClpC1-dependent degradation of model substrates was assayed with a set of mature ClpP1P2...
<p>ClpA is the chaperone subunit responsible for substrate recognition of the ClpAP ATP dependent pr...
The major chaperones identified in Escherichia coli that assist in protein folding include trigger f...
The ClpS adaptor collaborates with the AAA+ ClpAP protease to recognize and degrade N-degron substra...
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2009.This electronic versi...
<p>(A) Schematic representation of the inducible degradation system used to inducibly deplete ClpP2 ...
<p>(A) Quantitative PCR to determine transcript levels of over-represented proteins upon depletion o...
Targeted protein degradation is crucial for the correct function and maintenance of a cell. In bacte...
Staphylococcus aureus is an important pathogen, causing a wide range of infections including sepsis,...
ClpP is a self-compartmentalizing protease with crucial roles in bacterial and mitochondrial protein...
<p>(A) Schematic presentation of the Clp1 fusion gene, which is expressed under the control of <i>GA...
<p>A. Alignment of FtsZ residues 349 through 358 from the linker domain with the C-terminal ClpX rec...
Conditional degradation of proteins by ATP-dependent proteases is an important regulatory principe....
This article contains supporting information online at www.pnas.org/cgi/content/full/ 0910484106/DC...
Intracellular proteolysis is an essential regulatory process that affects cellular physiology. Since...
<p>ClpX and ClpC1-dependent degradation of model substrates was assayed with a set of mature ClpP1P2...
<p>ClpA is the chaperone subunit responsible for substrate recognition of the ClpAP ATP dependent pr...
The major chaperones identified in Escherichia coli that assist in protein folding include trigger f...
The ClpS adaptor collaborates with the AAA+ ClpAP protease to recognize and degrade N-degron substra...
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2009.This electronic versi...