The phosphorylation status of rpS6 plays no role in autophagy.

<p>(A) Immortalized Akt^tg and rpS6^P-/-;Akt^tg, MEFs were either untreated, incubated with 20 nM rapamycin, 50 μM chloroquine or both for 16 h. Cells were harvested and their cytoplasmic proteins were subjected to Western blot analysis with the indicated antibodies. (B) The signals of LC3-II, obtained in Western blot analyses of two independent experiments, as described in (A), were quantified and normalized to that tubulin. The fold change in the expression of LC3-II upon addition of rapamycin, chloroquine or both, was related to that of untreated cells, which was arbitrarily set at one.