Immunohistochemical staining for GDF9 in the rabbit ovaries of the mothers and F1- female offspring generations.

<p>Localization of the protein GDF9 in: (a) oocytes of the primordial and primary follicles in the VHT-treated mothers ovary; (b) oocytes and granulosa cells of the early antral follicle in theVHT- treated mothers ovary; (c) antral follicle- negative control of antibody; (d) oocytes of primary and primordial follicles in the ovary of F1 offspring born to treated mothers; (e) oocyte and cumulus-granulosa cells of the antral follicle in the ovary of F1 offspring born to treated mothers; (f) mouse ovary—positive control of antibody; (g) Original magnification: (a–f) × 20.