The relevance of low EVI genes to transient translational pausing and protein folding.

<p>(A, B) The RFP coverage of a representative gene with low EVI (A) and high TR (B) in HeLa cells, respectively, are shown with color bars. The highest RFP peaks are indicated by stars. The full length mRNAs are shown in thin lines, while their CDS regions are marked with thick lines. For more examples in all four analyzed cell lines, please refer to <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005901#pgen.1005901.s011" target="_blank">S6 Fig</a>. (C) The percentage distribution of the highest RFP peak of each gene relative to the start AUG codon. The highest RFP peaks more than 400 nucleotides are counted in the bin of 400. The <i>P</i>-values of Kolmogorov-Smirnov test between the low EVI genes and high TR genes as well as all genes in each cell line are indicated. The grey dashed lines mark the 240nt position. (D) Correlation between CDS length and EVI in the four cell lines. (E, F) The distribution of genes in HeLa cells according to their protein degradation rate constant (<i>k</i>_deg). Fractions of the genes with low EVI (E) and high TR (F) were indicated in red and blue bars, respectively. For comparison, the fraction distributions of total genes are shown in grey bars as background.