Specificity of PCR reaction in biological sample.

<p>Amplification specificity of <i>ama1</i> (<b>A</b>) and <i>hprt1</i> (<b>B</b>) was confirmed by comparing the average melting peaks for the respective primer sets using as template the plasmid DNA (colored line) or the DNA extracted from a biological sample consisting of a lymphocyte cell line, from bovine BV115, infected with the <i>T</i>. <i>parva</i> Muguga isolate (black line). Gel electrophoresis of the products was performed on a 2% agarose gel. The average peak melting temperature for <i>hprt1</i> was 74.9°C and 75.9°C, respectively, for the plasmid and the biological sample used as the source of DNA; for <i>ama1</i>, they were 79.8°C and 79.5°C, respectively for plasmid and biological sample.