CD69 expression in splenic lymphocytes.

<p>At 14 days after the second immunization, splenic lymphocytes of immunized mice were isolated and restimulated with the HA1-2 antigen (5 μg/mL) <i>in vitro</i>. The CD69 expression of splenic lymphocytes was determined by FACS analysis. (A) Representative images showing the CD69 expression in splenic lymphocytes. (B) The percentage of CD69⁺ CD4⁺ T cells in CD4⁺ T splenocytes, or CD69⁺ CD8⁺ T cells in CD8⁺ T splenocytes. (C) The percentage of CD69⁺CD4⁺ or CD69⁺CD8⁺ T cells in the total splenocytes. All data are presented as mean ± standard error of the mean; *<i>p</i> < 0.05, **<i>p</i> < 0.01, ***<i>p</i> < 0.001.