<p>Single cell measurements of fluorescence of strain CEN.PK 113-5D transformed with either of the following vectors: pCfB319 (episomal, 2μ, <i>URA3</i>), pCfB329 (single integrative at locus Chr. X-2, <i>URA3</i>), pCfB321 (multi-integrative, Ty4Cons based, <i>URA3</i>) and pCfB326 (multi-integrative, Ty4Cons, fusion <i>URA3</i>-degradation signal). Low (grey), mid (blue) and high (orange) expression levels were arbitrarily defined and are represented.</p
<p>(<b>A</b>) YFP measurements of clones with three different 3′ end sequences. Shown are YFP measur...
<p>A,C – target cell transduction rates; B,D – GFP fluorescence of virus producing wells. Bars in A ...
<p>Strain W303C (A1, A2), and isogenic mutants W303-Δ<i>syr2</i> (<i>sur2</i>) (B1, B2), W303-Δe<i>l...
<p>(A) Schematic illustration of the yeast strains expressing EGFP and mCherry. Time-lapse images of...
<p>Green fluorescence intensity is shown on the x-axes and red fluorescence intensity is shown on th...
<p>mCherry fluorescence was measured in the total culture sample. mCherry fluorescence was measured ...
<p>(A) Schematic illustration of the yeast strains expressing EYFP, ECFP, and mCherry. (B) Time-laps...
<p>FITC dye uptake without (A1, A2) and with FG08 (30 µg mL<sup>− 1</sup>) (B1, B2) or kanamycin B (...
<p>Yeast-based biosensors were either nontreated (NT, control) or exposed to increasing concentratio...
<p>(A-E) Cells infected with a mixture of the barcode-viruses at MOI 100 were monitored for 18 hours...
<p>Yeast strain BY4741 was transformed with pGK416-ZsGreen, pGK416-EGFP or pGK416 (empty vector). Al...
<p>J774A.1 cells were infected with various multiplicities of infection (MOI) of the BCG strains exp...
<p>Fluorescence is reported as a function of galactose level in culture (expressed as percent weight...
<p>The cells were incubated with 25/L natamycin in Synthetic wine at 28°C. After 30 (B); 60(C); 120(...
<p>(A) Schematic illustration of yeast-displayed gp140 that is C-terminally fused to Aga2. (B) Overl...
<p>(<b>A</b>) YFP measurements of clones with three different 3′ end sequences. Shown are YFP measur...
<p>A,C – target cell transduction rates; B,D – GFP fluorescence of virus producing wells. Bars in A ...
<p>Strain W303C (A1, A2), and isogenic mutants W303-Δ<i>syr2</i> (<i>sur2</i>) (B1, B2), W303-Δe<i>l...
<p>(A) Schematic illustration of the yeast strains expressing EGFP and mCherry. Time-lapse images of...
<p>Green fluorescence intensity is shown on the x-axes and red fluorescence intensity is shown on th...
<p>mCherry fluorescence was measured in the total culture sample. mCherry fluorescence was measured ...
<p>(A) Schematic illustration of the yeast strains expressing EYFP, ECFP, and mCherry. (B) Time-laps...
<p>FITC dye uptake without (A1, A2) and with FG08 (30 µg mL<sup>− 1</sup>) (B1, B2) or kanamycin B (...
<p>Yeast-based biosensors were either nontreated (NT, control) or exposed to increasing concentratio...
<p>(A-E) Cells infected with a mixture of the barcode-viruses at MOI 100 were monitored for 18 hours...
<p>Yeast strain BY4741 was transformed with pGK416-ZsGreen, pGK416-EGFP or pGK416 (empty vector). Al...
<p>J774A.1 cells were infected with various multiplicities of infection (MOI) of the BCG strains exp...
<p>Fluorescence is reported as a function of galactose level in culture (expressed as percent weight...
<p>The cells were incubated with 25/L natamycin in Synthetic wine at 28°C. After 30 (B); 60(C); 120(...
<p>(A) Schematic illustration of yeast-displayed gp140 that is C-terminally fused to Aga2. (B) Overl...
<p>(<b>A</b>) YFP measurements of clones with three different 3′ end sequences. Shown are YFP measur...
<p>A,C – target cell transduction rates; B,D – GFP fluorescence of virus producing wells. Bars in A ...
<p>Strain W303C (A1, A2), and isogenic mutants W303-Δ<i>syr2</i> (<i>sur2</i>) (B1, B2), W303-Δe<i>l...