Additional file 1: of Circulating miRNA signatures of early pregnancy in cattle

Determining haemolysis of bovine plasma samples. Samples with or without visible haemolysis were analysed to determine A) the ΔCq between miR-451 and miR-23, and B) optical density at 414 nm (as a measure of oxyhaemoglobin levels). ΔCq (C) and optical density (D) values for all plasma samples (n = 46) used for small RNA-sequencing and PCR array profiling, showing the absence of detectable haemolysis. Mean ± SEM is shown. (PDF 109 kb