Effect of salinity stress on <i>AdLEA</i> transgenic plants.

<p>(A and C) WT and transgenic seedlings after growth on MS medium supplemented with 200mM and 300mM NaCl respectively. (B and D) Individual seedlings morphology after growth on 200mM and 300mM NaCl respectively, depicting differences in root length between the seedlings. (E) Phenotypic differences in the leaf discs from WT and transgenic plant lines floated in dose-dependent concentrations (200mM and 300mM) of NaCl. (F) Graphical representation of root lengths in NaCl-treated seedlings after 15d and 9d growth on 200mM and 300mM NaCl respectively. (G) <b>C</b>hlorophyll content (μg mg^-1 FW) in the leaf discs after 72 h of treatment with NaCl. (H) Lipid peroxidation expressed as TBARS content (μmol g^-1 FW) in leaf discs after 72 h of treatment with NaCl. All the experiments were performed in triplicates and data represented as mean ± SD (n = 3; biological replicates). 11 seedlings per plate constitute one biological sample for seedling assay. 15 leaf discs from a single leaf constitute one biological sample for disc senescence assay. Statistical analysis was performed with two-way ANOVA (*P<0.05, **P<0.001).