Effects of ELF-EMF exposure on eNSCs differentiation.

<p>eNSCs were cultured in differentiation medium and exposed to ELF-EMF for 3 days. The cells were then fixed for Tuj1 (a neuronal marker) staining or cultured for another 3 days for GFAP (an astrocyte marker) staining. The mRNA expression of Tuj1 and GFAP was measured after ELF-EMF exposure for 3 days by real-time PCR. (<b>A</b>) Representative images of Tuj1⁺ and GFAP⁺ cells derived from eNSCs. Scale bar: 25 μm. (<b>B, D</b>) The graphs showed the statistical results of the percentage of Tuj1⁺ and GFAP⁺ cells in each condition. ** <i>p</i> < 0.01 vs. sham groups. (<b>C, E</b>) The mRNA expression of Tuj1 and GFAP. ** <i>p</i> < 0.01 vs. sham groups. For all experiments, the data are from five independent experiments and are presented as the mean ± SEM.