Summary of <i>in vivo</i> study timeline and muscle contractility assay.

<p>A) Schematic representation of the dietary intervention study performed on 48 C57BL/6nTac male mice. All mice were 19 months old at the time of sacrifice for contraction studies. B) Representative tracing of force data from the <i>ex vivo</i> contractility assay obtained from one muscle (<i>X-axis</i>: Time; <i>Y-axis</i>: Force). Equilibration, fatigue, and recovery from fatigue protocols are performed using alternating high (80 Hz) and low (20 Hz) frequencies of stimulation. <i>Inset</i>: Magnified view of fatiguing stimulations using alternating high (80 Hz) and low (20 Hz) stimulation frequencies.