Sequence alignment of <i>Ld</i>RpiB amino acid sequence with amino acid sequence of <i>T</i>. <i>cruzi</i> RpiB and <i>H</i>. <i>sapiens</i> RpiA using Clustal W.

<p>Black dot represents the amino acids targeted for site directed mutagenesis, black box represents the amino acids present in the substrate binding pocket of <i>T</i>. <i>cruzi</i> and red box represents the other conserved amino acids.