Quantitative RT-PCR determination of transcripts in B-13, B-13/H cells and comparison to rat liver and pancreas levels.

<p>Time course for the expression of amylase mRNA (<b>A</b>) or Cebp-α (-■-), Cebp- β (-●-), albumin (-▲-) and Cyp2e1 (-♦) mRNAs (<b>B</b>) in B-13 cells after treatment with 10nM DEX. Data are mean and standard deviation of three independent experiments. B-13 cells treated with 0.1% (v/v) ethanol (i.e. vehicle control) had minimal effect on all transcripts and are not shown for clarity. After 3–4 days, B-13 cells unexposed to DEX required sub-culturing since they continued to proliferate to avoid cell death. Therefore, they were not appropriate controls for B-13 cells treated with DEX after this time since DEX treatment resulted in a cessation of proliferation and sub-culture was not required. <b>C—E,</b> Expression levels of the indicated 18S rRNA-normalised transcript, relative to the levels present in B-13 cells, data are the mean and standard deviation of at least 3 separate cell samples/rat tissue samples.