MOESM1 of Bortezomib-mediated downregulation of S-phase kinase protein-2 (SKP2) causes apoptotic cell death in chronic myelogenous leukemia cells

Additional file 1: Figure S1. Bortezomib-mediated induction of early (Annexin +ive and PI −ive cells), late apoptosis (Annexin +ive and PI +ive cells) and necrosis fractions (Annexin −ive and PI +ive cells) in K562 (A) and AR230 (B) cells. Cells were treated with 10, 25 and 50 nM of bortezomib for 24 h and cells were subsequently stained with flourescein-conjugated annexin-V and propidium iodide (PI) and analyzed by flow cytometry. The graph displays the mean ± SD (standard deviation) of three independent experiments for all the doses. *** p < 0.001. Bortezomib mediated induction apoptosis in K562 (C) and AR230 (D) cells. The percentage of apoptotic cells relative to control population is shown. The graph displays the mean ± SD (standard deviation) of three independent experiments for all the doses