Autophagy inhibition and analysis of apoptosis in control and patients´ fibroblasts by flow cytometry.

<p>Cells were incubated for 48h with 20 mM of 3-MA. Data represent mean values ± standard deviation from two experiments performed by triplicate (*<i>P</i><0.05; **<i>P</i><0.01; ***<i>P</i><0.001). (A) Percentage of annexinV-positive apoptotic cells in patients and control cells relative to total cell number per sample. (B) Percentage of viability in patients and control cells relative to total cell number per sample. Dot blots from a representative experiment of apoptosis levels are shown.