The expansion of CD5⁺ B cells, NK and NKT cells is impacted by the loss of IL-10.

<p>The frequency of splenic and peritoneal cells was measured in 4 month old mice by flow cytometry. Representative gating and results for splenic CD5⁺ B cell (A), peritoneal CD5⁺ B cell (B), NK cell (C) and NKT cell frequencies (D). Each point represents a single mouse, with the lines for each group representing the median. Statistical analyses were carried out using a Mann-Whitney <i>U</i> test between homozygous and IL-10 knockout animals of the same genetic background. * P < 0.05, ** P < 0.01, ***P <0.001.