Pam3CSK4 pretreatment protects mice from MRSA infection.

<p>KM mice were anesthetized (i.p.) with 100 μl 10% chloralhydrate (CHO), then inhaled Pam3CSK4 (0, 25, 50, 100 μg /mice) in 70 μl normal saline through nasal cavity. 24 h later, mice inhaled MRSA. Survival curve of mice pretreated with Pam3CSK4 versus normal saline after intranasal infection with 1.5x10⁹ cfu MRSA(n = 13) (A). Bacterial burden in lung (B) and bronchus (C) was calculated 12 h after pulmonary infection with MRSA (2×10⁸ cfu/mice, n = 6). Note: *P<0.05; **P<0.01 <i>versus</i> normal saline-treated mice.