Enabling Metabolomics Based Biomarker Discovery Studies Using Molecular Phenotyping of Exosome-Like Vesicles - Fig 5

<p><b>Panel A. TGF-beta induces epithelial to mesenchymal transition (EMT) in PANC1 cells</b>. Real Time PCR analysis showed a decrease of the epithelial marker E-cadherin (p-v<0.05) and an increase of the mesenchymal markers N-Cadherin and Vimentin (p-v<0.05) expression after TGF-β treatment in PANC1 cells. <b>Panel B</b>. <b>Principal Component Analysis (PCA) of exosomal metabolome for MS negative ionization mode showing the separation between the two study groups</b>. TGF-β treated (T) and control (C) PANC1 cells. We analyzed three replicates per condition. The x-axis shows interclass separation while y-axis illustrates the intra-class variability on Y-axis.