Purity of CD4+ T cells analyzed by flow cytometry.

Quantification of cells with specific phenotypes II: Determination of CD4 expression level on reconstituted lyophilized human PBMC labelled with anti‐CD4 FITC antibody

Wang
L.
Stebbings
R.
Gaigalas
A. K.
Sutherland
J.
Kammel
M.
John
M.
Roemer
B.
Kuhne
M..
Schneider
R.
Braun
M
Engel
A.
Dinesh K. Dikshit
D.
Abbasi F.
Marti
G.
Sassi M
Revel
L.
Kim
S.
Marc-Olivier Baradez
M.O.
Lekishvili
T.
Marshall
D.
Whitby
L.
Wang
J.
Ost
V.
Vonsky
M. and Neukammer
J.

January 2015

This report focuses on the characterization of CD4 expression level in terms of equivalent number of...

Flow cytometric analysis of thymocytes from Pbx1NT TG mice (TG) and their wild-type controls (WT).

Dmitry Penkov (126478)
Martina Palazzolo (377442)
Anna Mondino (377443)
Francesco Blasi (61418)

February 2013

(A) Thymocytes were stained with PE-conjugated anti-CD4, CyChrome-conjugated anti-CD8, and FITC-c...

Differentiation of CD4+CD25- naïve T cells from peripheral blood of healthy subjects.

Shuguang Xu (538163)
Jingzhi Zhu (538164)
Zhiyong Wu (515330)

March 2014

(A) CD4+CD25- naïve T cells treated with negative control medium, condition medium from SK-BR-3 V...

The purity of CD4+ T cells and the GFP-transfection efficiency in CD4+ T cells.

Rui Yao (205074)
Yu-Lan Ma (302641)
Wei Liang (26352)
Huan-Huan Li (302645)
Zhi-Jun Ma (302648)
Xian Yu (302651)
Yu-Hua Liao (168020)

February 2013

A. CD4+ T cells were purified by magnetic cell sorting (MACS). The purity of CD4+...

Flow cytometric analysis of mononuclear cells in IL2RG KO pigs.

Masahito Watanabe (406126)
Kazuaki Nakano (406125)
Hitomi Matsunari (406127)
Taisuke Matsuda (406128)
Miki Maehara (406130)
Takahiro Kanai (406131)
Mirina Kobayashi (406133)
Yukina Matsumura (468456)
Rieko Sakai (468457)
Momoko Kuramoto (406134)
Gota Hayashida (406132)
Yoshinori Asano (468458)
Shuko Takayanagi (468459)
Yoshikazu Arai (406135)
Kazuhiro Umeyama (406136)
Masaki Nagaya (406139)
Yutaka Hanazono (406140)
Hiroshi Nagashima (406141)

October 2013

(A) Flow cytometric analysis of T, B, and NK cells in the peripheral blood of IL2RG KO pig...

The purity of peripheral blood enriched CD4+ and CD8+ T cell populations after magnetic beads isolation.

Mahmoud Nateghi Rostami (361016)
Hossein Keshavarz (361017)
Rosita Edalat (361018)
Abdolfattah Sarrafnejad (361019)
Tahereh Shahrestani (361020)
Fereidoun Mahboudi (361021)
Ali Khamesipour (62371)

February 2013

CD4+ (A) and CD8+ (B) lymphocytes were isolated from a cell suspension of P...

Purity of resting CD3+ T cell population.

Rudolf Lichtenfels (149675)
Gunter Rappl (107197)
Andreas A. Hombach (169001)
Christian V. Recktenwald (149713)
Sven P. Dressler (149700)
Hinrich Abken (107201)
Barbara Seliger (149734)

April 2012

CD3+ T cells were isolated from peripheral blood by negative selection procedures usin...

Flow cytometric analysis of human peripheral blood CD4 T cells.

Sriram Narayanan (364941)
Rodolfo Silva (364942)
Giovanna Peruzzi (264239)
Yelina Alvarez (210789)
Venkateswara R. Simhadri (364944)
Karen Debell (364945)
John E. Coligan (364946)
Francisco Borrego (364947)

February 2013

In the upper panel, freshly isolated CD4 T cells were labeled with anti-CD4, -CD45RO, and -CD300a...

Flow cytometric analysis of surface marker expression on pMDMCs.

Cesar Caballero
Theresa Rathbun
Deryl Troyer
Duane Davis

February 2023

.Cell suspensions were stained with monoclonal antibodies indicated in filled histograms: CD 14 (A),...

Preparation and characterization of monoclonal antibodies recognizing two CD4 isotypes of Microminipigs.

Shino Ohshima
Tatsuya Matsubara
Asuka Miyamoto
Atsuko Shigenari
Noriaki Imaeda
Masaki Takasu
Masafumi Tanaka
Takashi Shiina
Shingo Suzuki
Noriaki Hirayama
Hitoshi Kitagawa
Jerzy K Kulski
Asako Ando
Yoshie Kametani

January 2020

Cluster of differentiation 4 (CD4) molecule expressed on the leukocytes is known to function as a co...

Fine phenotyping of swine immune cells by multicolor flow cytometry

Blanc, Fany
Prevost-Blondel, Armelle
Piton, Guillaume, G.
Bouguyon, Edwige
Leplat, Jean Jacques
Andreoletti, Fabrice
Bourneuf, Emmanuelle, E.
Bertho, Nicolas
Vincent-Naulleau, Silvia, S.

March 2018

National audienceImmunological research in a species such as the pig is of great importance for its ...

Flow cytometric analysis of CD4+ and CD8+ T cells of differently treated and untreated infected BALB/c mice.

Mohammad Islamuddin (682421)
Garima Chouhan (682422)
Abdullah Farooque (632787)
Bilikere S. Dwarakanath (199967)
Dinkar Sahal (291989)
Farhat Afrin (278211)

January 2015

Splenocytes (2 × 106) at 10 days post-treatment were stained with anti-CD4+...

Flow cytometry methodology (see also references 20, 52).

Syed R. Gilani (367562)
Louis J. Vuga (367563)
Kathleen O. Lindell (367564)
Kevin F. Gibson (172460)
Jianmin Xue (355591)
Naftali Kaminski (27713)
Vincent G. Valentine (355606)
Emily K. Lindsay (367565)
M. Patricia George (367566)
Chad Steele (13079)
Steven R. Duncan (355610)

February 2013

Aliquots of fresh, live peripheral blood mononuclear cells (PBMNC) were stained with anti-CD4-all...

Simultaneous expression of CD4 and CD8 antigens by a substantial proportion of resting porcine T lymphocytes

Saalmüller, Armin
Reddehase, Matthias J.
Bühring, Hans-Jörg
Jonjic, Stipan
Koszinowski, Ulrich H.

January 1987

The existence of four subpopulations in resting porcine T lymphocytes is documented. In addition to ...

Flow cytometric characterization of CD3+ T cells ((A) CD4+ T cells, (B) CD8+ T cells, (C) CD4+CD8+ T cells) in PBMCs culture of young (10 wk) and aged (5.5 y) pigs vaccinated with PRV-CpG vaccine or PRV alone in response to PRV activation ex vivo.

Feiping Ming (421556)
Jun Yang (2084)
Pinpin Chu (421557)
Miaopeng Ma (421558)
Juqing Shi (421559)
Haiming Cai (421560)
Chaoyuan Huang (421561)
Huazhou Li (421562)
Zhenggu Jiang (421563)
Houguang Wang (421564)
Weifang Wang (421565)
Shuiqing Zhang (421566)
Linghua Zhang (421567)

June 2013

Legend: Samples collected 3 pools in each group were used for CD3/CD4/CD8/CD45RO and CD3/CD4/CD8/...

Quantification of cells with specific phenotypes II: Determination of CD4 expression level on reconstituted lyophilized human PBMC labelled with anti‐CD4 FITC antibody

Wang
L.
Stebbings
R.
Gaigalas
A. K.
Sutherland
J.
Kammel
M.
John
M.
Roemer
B.
Kuhne
M..
Schneider
R.
Braun
M
Engel
A.
Dinesh K. Dikshit
D.
Abbasi F.
Marti
G.
Sassi M
Revel
L.
Kim
S.
Marc-Olivier Baradez
M.O.
Lekishvili
T.
Marshall
D.
Whitby
L.
Wang
J.
Ost
V.
Vonsky
M. and Neukammer
J.

January 2015

This report focuses on the characterization of CD4 expression level in terms of equivalent number of...

Flow cytometric analysis of thymocytes from Pbx1NT TG mice (TG) and their wild-type controls (WT).

Dmitry Penkov (126478)
Martina Palazzolo (377442)
Anna Mondino (377443)
Francesco Blasi (61418)

February 2013

(A) Thymocytes were stained with PE-conjugated anti-CD4, CyChrome-conjugated anti-CD8, and FITC-c...

Differentiation of CD4+CD25- naïve T cells from peripheral blood of healthy subjects.

Shuguang Xu (538163)
Jingzhi Zhu (538164)
Zhiyong Wu (515330)

March 2014

(A) CD4+CD25- naïve T cells treated with negative control medium, condition medium from SK-BR-3 V...

The purity of CD4+ T cells and the GFP-transfection efficiency in CD4+ T cells.

Rui Yao (205074)
Yu-Lan Ma (302641)
Wei Liang (26352)
Huan-Huan Li (302645)
Zhi-Jun Ma (302648)
Xian Yu (302651)
Yu-Hua Liao (168020)

February 2013

A. CD4+ T cells were purified by magnetic cell sorting (MACS). The purity of CD4+...

Flow cytometric analysis of mononuclear cells in IL2RG KO pigs.

Masahito Watanabe (406126)
Kazuaki Nakano (406125)
Hitomi Matsunari (406127)
Taisuke Matsuda (406128)
Miki Maehara (406130)
Takahiro Kanai (406131)
Mirina Kobayashi (406133)
Yukina Matsumura (468456)
Rieko Sakai (468457)
Momoko Kuramoto (406134)
Gota Hayashida (406132)
Yoshinori Asano (468458)
Shuko Takayanagi (468459)
Yoshikazu Arai (406135)
Kazuhiro Umeyama (406136)
Masaki Nagaya (406139)
Yutaka Hanazono (406140)
Hiroshi Nagashima (406141)

October 2013

(A) Flow cytometric analysis of T, B, and NK cells in the peripheral blood of IL2RG KO pig...

The purity of peripheral blood enriched CD4+ and CD8+ T cell populations after magnetic beads isolation.

Mahmoud Nateghi Rostami (361016)
Hossein Keshavarz (361017)
Rosita Edalat (361018)
Abdolfattah Sarrafnejad (361019)
Tahereh Shahrestani (361020)
Fereidoun Mahboudi (361021)
Ali Khamesipour (62371)

February 2013

CD4+ (A) and CD8+ (B) lymphocytes were isolated from a cell suspension of P...

Purity of resting CD3+ T cell population.

Rudolf Lichtenfels (149675)
Gunter Rappl (107197)
Andreas A. Hombach (169001)
Christian V. Recktenwald (149713)
Sven P. Dressler (149700)
Hinrich Abken (107201)
Barbara Seliger (149734)

April 2012

CD3+ T cells were isolated from peripheral blood by negative selection procedures usin...

Flow cytometric analysis of human peripheral blood CD4 T cells.

Sriram Narayanan (364941)
Rodolfo Silva (364942)
Giovanna Peruzzi (264239)
Yelina Alvarez (210789)
Venkateswara R. Simhadri (364944)
Karen Debell (364945)
John E. Coligan (364946)
Francisco Borrego (364947)

February 2013

In the upper panel, freshly isolated CD4 T cells were labeled with anti-CD4, -CD45RO, and -CD300a...

Flow cytometric analysis of surface marker expression on pMDMCs.

Cesar Caballero
Theresa Rathbun
Deryl Troyer
Duane Davis

February 2023

.Cell suspensions were stained with monoclonal antibodies indicated in filled histograms: CD 14 (A),...

Preparation and characterization of monoclonal antibodies recognizing two CD4 isotypes of Microminipigs.

Shino Ohshima
Tatsuya Matsubara
Asuka Miyamoto
Atsuko Shigenari
Noriaki Imaeda
Masaki Takasu
Masafumi Tanaka
Takashi Shiina
Shingo Suzuki
Noriaki Hirayama
Hitoshi Kitagawa
Jerzy K Kulski
Asako Ando
Yoshie Kametani

January 2020

Cluster of differentiation 4 (CD4) molecule expressed on the leukocytes is known to function as a co...

Fine phenotyping of swine immune cells by multicolor flow cytometry

Blanc, Fany
Prevost-Blondel, Armelle
Piton, Guillaume, G.
Bouguyon, Edwige
Leplat, Jean Jacques
Andreoletti, Fabrice
Bourneuf, Emmanuelle, E.
Bertho, Nicolas
Vincent-Naulleau, Silvia, S.

March 2018

National audienceImmunological research in a species such as the pig is of great importance for its ...

Flow cytometric analysis of CD4+ and CD8+ T cells of differently treated and untreated infected BALB/c mice.

Mohammad Islamuddin (682421)
Garima Chouhan (682422)
Abdullah Farooque (632787)
Bilikere S. Dwarakanath (199967)
Dinkar Sahal (291989)
Farhat Afrin (278211)

January 2015

Splenocytes (2 × 106) at 10 days post-treatment were stained with anti-CD4+...

Flow cytometry methodology (see also references 20, 52).

Syed R. Gilani (367562)
Louis J. Vuga (367563)
Kathleen O. Lindell (367564)
Kevin F. Gibson (172460)
Jianmin Xue (355591)
Naftali Kaminski (27713)
Vincent G. Valentine (355606)
Emily K. Lindsay (367565)
M. Patricia George (367566)
Chad Steele (13079)
Steven R. Duncan (355610)

February 2013

Aliquots of fresh, live peripheral blood mononuclear cells (PBMNC) were stained with anti-CD4-all...

Simultaneous expression of CD4 and CD8 antigens by a substantial proportion of resting porcine T lymphocytes

Saalmüller, Armin
Reddehase, Matthias J.
Bühring, Hans-Jörg
Jonjic, Stipan
Koszinowski, Ulrich H.

January 1987

The existence of four subpopulations in resting porcine T lymphocytes is documented. In addition to ...

Flow cytometric characterization of CD3+ T cells ((A) CD4+ T cells, (B) CD8+ T cells, (C) CD4+CD8+ T cells) in PBMCs culture of young (10 wk) and aged (5.5 y) pigs vaccinated with PRV-CpG vaccine or PRV alone in response to PRV activation ex vivo.

Feiping Ming (421556)
Jun Yang (2084)
Pinpin Chu (421557)
Miaopeng Ma (421558)
Juqing Shi (421559)
Haiming Cai (421560)
Chaoyuan Huang (421561)
Huazhou Li (421562)
Zhenggu Jiang (421563)
Houguang Wang (421564)
Weifang Wang (421565)
Shuiqing Zhang (421566)
Linghua Zhang (421567)

June 2013

Legend: Samples collected 3 pools in each group were used for CD3/CD4/CD8/CD45RO and CD3/CD4/CD8/...

Quantification of cells with specific phenotypes II: Determination of CD4 expression level on reconstituted lyophilized human PBMC labelled with anti‐CD4 FITC antibody

Wang
L.
Stebbings
R.
Gaigalas
A. K.
Sutherland
J.
Kammel
M.
John
M.
Roemer
B.
Kuhne
M..
Schneider
R.
Braun
M
Engel
A.
Dinesh K. Dikshit
D.
Abbasi F.
Marti
G.
Sassi M
Revel
L.
Kim
S.
Marc-Olivier Baradez
M.O.
Lekishvili
T.
Marshall
D.
Whitby
L.
Wang
J.
Ost
V.
Vonsky
M. and Neukammer
J.

January 2015

This report focuses on the characterization of CD4 expression level in terms of equivalent number of...

Flow cytometric analysis of thymocytes from Pbx1NT TG mice (TG) and their wild-type controls (WT).

Dmitry Penkov (126478)
Martina Palazzolo (377442)
Anna Mondino (377443)
Francesco Blasi (61418)

February 2013

(A) Thymocytes were stained with PE-conjugated anti-CD4, CyChrome-conjugated anti-CD8, and FITC-c...

Differentiation of CD4+CD25- naïve T cells from peripheral blood of healthy subjects.

Shuguang Xu (538163)
Jingzhi Zhu (538164)
Zhiyong Wu (515330)

March 2014

(A) CD4+CD25- naïve T cells treated with negative control medium, condition medium from SK-BR-3 V...

Purity of CD4<sup>+</sup> T cells analyzed by flow cytometry.

Abstract

Extracted data

Purity of CD4<sup>+</sup> T cells analyzed by flow cytometry.

Abstract

Extracted data

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