PD-1 and CTLA-4 expressions on FVS 510-/Lymph/CD3+/CD4+ and CD8+ T cells.

<p>(A) Representative histogram of PD-1 and CTLA-4 expressions on CD4+ T cells of uninfected, OB- and OS-infected mice after 60 days of infection. Median fluorescence intensities (MFIs) of PD-1 on (B) CD4+ and (C) CD8+ T cells, and (D) CTLA-4 on CD4+ and (E) CD8+ T cells of uninfected, OB- and OS-infected mice. Mean fluorescence intensity is used in (D) as median shows negative fluorescence values. Logarithm with base 10 is used for (B) and (D) for improved illustration purposes. Data are pooled from two independent experiments (B-E; n = 6 per group). Box plots show the median value (line), the interquartile range (box), and the minimum and maximum values (whiskers). Correlation analyses between CD4+ T-cell frequency and PD-1 expression on (F) CD4+ and CD8+ T cells, and between PD-1 expression on CD4+ and CD8+ T cells. Data are pooled from all uninfected, OB, and OS-infected mice from two independent experiments (F; total n = 18). <i>P</i> values calculated using Mann-Whitney U test (B–E) and Spearman’s Rank Order Coefficient (F). *<i>P</i><0.025, **<i>P</i><0.005, ***<i>P</i><0.0005 after Bonferroni correction for 2 comparisons.