FSK-mediated attenuation of UPR is accompanied by an increase of membrane protein glycosylation.

<p>(<b>A-B)</b> UPR-related transcriptional data from microarray (A) and proteomic (B) data. (<b>C-G)</b> All the analyses are referred to Transformed cells and were performed at 72h (C-E) or 96h (F-G) of culture. (<b>C)</b> qPCR analysis of UPR mRNA levels in FSK-treated cells with respect to untreated cells. (<b>D)</b> Western blot analysis of UPR-related proteins. (<b>E)</b> Schematic representation of the HBP in which the expression levels of some HBP-related mRNAs have been indicated. The data are the ratio TF/T and are represented in color code (red up-regulated; yellow unchanged; green down-regulated). (<b>F)</b> FACS analysis of live cells stained with fluorochrome-conjugated ConcanavalinA. (<b>G)</b> Western blot analysis using a specific anti O-GlcNAc antibody and densitometric analysis of the film. All data represent the average of different experiments (n>3).