Changes in nuclear levels of pCREB after BDNF treatment in <i>Jacob/Nsmf</i> ko and wt hippocampal neurons.
- Christina Spilker (80108)
- Sven Nullmeier (3179766)
- Katarzyna M. Grochowska (3179772)
- Anne Schumacher (142704)
- Ioana Butnaru (3179769)
- Tamar Macharadze (518874)
- Guilherme M. Gomes (3179754)
- PingAn Yuanxiang (3179757)
- Gonca Bayraktar (3179760)
- Carolin Rodenstein (3179742)
- Carolin Geiseler (3179745)
- Angela Kolodziej (3179751)
- Jeffrey Lopez-Rojas (3179739)
- Dirk Montag (106874)
- Frank Angenstein (325892)
- Julia Bär (577169)
- Wolfgang D’Hanis (3179736)
- Thomas Roskoden (3179733)
- Marina Mikhaylova (80099)
- Eike Budinger (41785)
- Frank W. Ohl (41784)
- Oliver Stork (50726)
- Ana C. Zenclussen (3179763)
- Anna Karpova (80098)
- Herbert Schwegler (3179748)
- Michael R. Kreutz (194048)
Publication date
March 2016
DOIAbstract
<p>(A) DIV 10 hippocampal neurons were treated and stained as indicated. (B) Quantification revealed that in Jacob-deficient neurons the nuclear pCREB level is lower under basal conditions. After application of BDNF, nuclear amounts of pCREB significantly increased in neurons from both genotypes. Interestingly, application of the NMDAR-antagonist AP5 normalizes the differences in nuclear pCREB immunostaining intensity in wt and ko neurons. Co-application of AP5 with BDNF had only in wt neurons a statistically significant effect. Two-way ANOVA with Tukey posttest ***p<0.001; **p<0.01, *p<0.05. Scale bars indicate 20μm.</p
Add to ORKG