Expression of Nrf2 in renal tissues by immunohistochemistry.

<p>(A) Renal tissue sections stained by immunohistochemistry (500× magnification). Little brown yellow staining in cytoplasm of renal tubular epithelial cells was observed in the control group at all time points. Many positive nuclear staining was observed in group PHC at 1 h and 6 h. Some positive nuclear staining was observed in group AKI at 1 h and 24 h, in group ZnPP at 1 h and 6 h and in group PHC+ZnPP at 6 h and 24 h. Scale bar corresponds to 100 μm. (B) Semi-quantitative evaluation of Nrf2 expression represented as IOD/area. Each bar represents mean ± SD. Compared with simultaneous group: * <i>P</i><0.01 <i>vs</i>. Control; # <i>P</i><0.01 <i>vs</i>. AKI; ^ <i>P</i><0.01 <i>vs</i>. PHC.