Surface Plasmon resonance of calumenin with different divalent ions.

<p>SPR analysis of Ca²⁺-binding to calumenin. Sensorgrams obtained at 8 different ion concentrations (a) in quadruplicate were processed with the Biacore analysis software. The curve (b) provided a K_d,Ca value of 201 ± 13 μM (n = 32) for the interaction between Ca²⁺ and immobilised calumenin.